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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which results in chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was identified to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a important position in marketing mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two additional splice variants ended up noted but detailed nucleotide sequence data isn't nonetheless obtainable.
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Blocking peptides are peptides that bind exclusively to the target antibody and block antibody binding. These peptide typically incorporates the epitope recognized with the antibody.
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This manual outlines further doable optimization of cellular and immunoassay parameters to make sure the very best benefits are...
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This tutorial outlines more doable optimization of mobile and immunoassay parameters to make certain the absolute best effects are...
Antibodies bound to the blocking peptide not bind on the epitope about the focus on protein. By comparing the staining get more info with the blocked antibody vs . the antibody by itself, you can see which staining is precise.
Concentrate on Information Cyclin E belongs to the very conserved cyclin household, whose customers are characterized by a remarkable periodicity in protein abundance through the mobile cycle. Cyclins functionality as regulators of CDK kinases. Distinctive cyclins show unique expression and degradation designs which contribute towards the temporal coordination of each mitotic function. Cyclin E forms a posh with and functions being a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S changeover. This protein accumulates on the G1-S phase boundary and is particularly degraded as cells progress through S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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